The traditional method of treating for tracheal mites is the use of menthol crystals which are placed on the top of the hive. As the menthol evaporates, it enters the trachea of the bees thus killing the mites.
An alternative treatment is a mixture of powdered sugar and Crisco. The bees go after the sugar and get covered with the Crisco. This interferes with the mite's ability to detect the young bees. As a result, the mite remains on the older bee and eventually dies with the bee. Be cautious with this treatment because it can attract hive beetles.
If you suspect Tracheal mites then you want to treat for the mites and then requeen.